1x Transfer Buffer. 1x buffer should be ph 72 do not adjust with acid or base. Place the cassette in the transfer tank and place an ice block in the tank.
This product supplies enough 10x material to make 10 liters of 1x solution. Ponceau s staining solution. Prepare 1x transfer buffer b.
Add deionized water to 10l.
50ml of trisgly buffer stock 100ml 10 methanol 850ml water 2x sds sample buffer. Directions for 1x transfer buffer. Remove the precut nitrocellulose membrane and cut 6 sheets 3 for each side of 3 mm whatman filter paper to the same size as the precut membrane usually 3 x 4. Mix 100 ml of 10x transfer buffer.