Western Blot Transfer. Fast transfer in as little as 3 min highest efficiency transfer. Compare our wet tank semi dry and dry western blot transfer systems.
Residual unbound antibodies or other reagents remaining between steps can produce a high background. Western blot transfer and total protein imaging. Keep on ice throughout the western blot process.
Preassembled trans blot turbo transfer packs are optimized for superior blot transfer.
Briefly rinse the blot in water and stain it with ponceau s solution to check the transfer quality. Also dry or seminary transfer work well reducing waste. Transfer can also be done at 100 v for 30 min2 hr but the method needs to be optimized for proteins of different sizes. Rinse off the ponceau.