Western Blot Transfer Voltage. I transfer the proteins on nitrobind cast pure nitrocellulose membrane cat noep2hy00010 gvs maine with 175 volts or 300 mamp for 75 90 minutes depending on the size of protein. Posted by the protein man on jan 20 2017 122753 pm.
The next stage is to transfer the proteins from the gel onto a membrane. In rapid methods amperage is held constant and voltage is limited to a maximum of 25v. Use higher voltage 140 v for stacking gel figure 5a and b.
Equilibrate gel in transfer buffer for 10 minutes prior to transfer.
Ad preferred choice of blocking buffer and antibody diluent supplied as a lyophilized powder. Ad explore our extensive portfolio of western blot transfer systems. 1 hour at a constant current 125 macm2. Compare our wet tank semi dry and dry western blot transfer systems.