Western Blot Transfer Protocol. Ad explore our extensive portfolio of western blot transfer systems. In 19791 and re named western blot by burnette wn 19812 there have been many modifications that assist better transfer of particular types of proteins.
Selection of transfer protocols using the navigation and selection buttons is simple and the. 1 dissolve tris base and glycine together in 18 l of ddh. Western blotting principle usually involves two major processes namely sds polyacrylamide gel electrophoresis and protein blotting and testing.
Do not use acid or base to adjust ph.
O to a final volume of 2 l. Directions for 10x transfer buffer. The blot should be on the cathode and the gel on the anode. Assemble the transfer sandwich and make sure no air bubbles are trapped in the sandwich.