Transfer Buffer. Directions for 10x transfer buffer. Such devices should provide constant high current.
This transfer buffer has both low ionic strength and low conductivity which is optimal for tank wet blotting and for some semi dry apparatuses. Add transfer buffer to the apparatus and ensure that the sandwich is covered with the buffer. Open in a separate window figure 7 transfer should be done on ice transfer for 90 minutes figure 8.
Transfer is completed in 10 or 20 minutes using a standard semi dry or wet transfer apparatus respectively.
10 sheets 8 cm x 10 cm the first pvdf transfer membrane for western blotting that wets out in transfer buffer or water. 1 step transfer buffer is compatible with power blotter and other protein semi dry transfer devices when they are paired with a suitable high current power supply. Apparatus used is biorad mini transblot tankwet transfer. Tris glycine transfer buffer 10x is a commonly used western blot buffer for the electrotransfer of proteins from sds page gels to nitrocellulose or pvdf membranes.