Transfer Buffer Recipe. Actively helping customers employees and the global community during the coronavirus sars cov 2 outbreak. Assemble the transfer sandwich and make sure no air bubbles are trapped in the sandwich.
Collapse all expand all properties storage room temperature applications reconstitute with 900 ml h 2 othen add 100 ml of methanol or ethanol per bag. 576 g tris base 295 g glycine 200 ml methanol. Mix well and filter.
In many cases ethanol can be substituted for methanol in the transfer buffer with minimal impact on transfer efficiency.
30 g tris base 144 g glycine 200 ml methanol. 10 ml meoh 30 mg 4 chloro 1 naphthol. Mix 100 ml of 10x transfer buffer 100 ml of methanol and 800 ml of ddh. Reduces overall background minimizes non specific signals often seen with ecl detection.