Trans Blot Turbo Transfer. For western blotting we are using bio rad trans blot turbo transfer system by using semi dry consumables. Many factors affect the transfer of proteins from a gel to a membrane.
Economical transfer option for high throughput labs. Transfer buffer used was bjerrum schafer nielsen buffer 48 mm tris 39 mm glycine ph 92 containing 20 methanol containing 01 sds. The trans blot turbo instrument and reagents are designed to provide fast efficient and reproducible western blots of protein gels in as little as 3 min.
Using the wypallx60 and the trans blot turbo system i see a pattern of the filter paper etched on the blot after transfer and ponceau s stain.
Bio rads trans blot turbo system accelerates the semi dry blotting process without sacrificing performance. The trans blot turbo system is a rapid protein transfer apparatus that can transfer protein to membrane in as little as 3 minutes. Bio rads trans blot turbo system accelerates the semi dry blotting process without sacrificing performance. Each convenient transfer pack contains the necessary materials to efficiently transfer a single mini gel 70 x 85 cm or.