Forster Resonance Energy Transfer. Fret is based on non radiative transfer of energy between an excited donor molecule and an acceptor molecule. Förster fluorescence resonance energy transfer fret resonance energy transfer ret or electronic energy transfer eet is a mechanism describing energy transfer between two chromophores1 a donor chromophore initially in its electronic excited state may transfer energy to an acceptor chromophore through nonradiative dipoledipole coupling2 the efficiency of this energy.
The förster energy transfer is the phenomenon that an excited donor transfers energy not an electron to an acceptor group through a non radiative process. Genetically encoded förster resonance energy transfer fret based probes allow a sensitive readout for different or specific parameters in the living cell. Here we discuss förster resonance energy transfer fret in liposomes.
It is an absorption of electromagnetic radiation by one molecule the donor followed by resonance interaction with another molecule the acceptor.
Förster resonance energy transfer fret is a widely used single molecule technique for measuring distances within and between molecules 1 2. 563 energy transfer to metal nanoparticles fret nset dmpet npilm etc 148 564 other transfer mechanisms 150 5641 electron exchange energy transfer dexter transfer 151 5642 charge transfer marcus theory 152 5643 plasmon coupling 153 5644 singlet oxygen diffusion 154 57 summary and outlook 155 references 156. Förster resonance energy transfer fret based small molecule sensors and imaging agents. Forster resonance energy transfer occurs between two appropriately positioned fluorophores only when the distance separating them is less than 10 nanometers.