Direct Electron Transfer. The organometallic compound ferrocene and its derivatives have proved particularly effective in this role. Instead direct electron transfer between archaea and bacteria mediated by large multi haem cytochromes produced by anme 2 archaea is a central mechanism of their interaction.
As opposed to this multistep scenario a direct interfacial electron transfer can vertically excite charges from their initial electronic states at donor molecules to the unoccupied electronic states of acceptors such as charge transfer states in a single step 7 13 1520. To study the direct electron transfer det of the multicofactor enzyme cellobiose dehydrogenase cdh in regard to its orientation on an electrode surface a recently published maleimide based immobilization method was used in combination with site directed mutagenesis to establish different orientations on an electrode surface. Direct electron transfer of other active enzymes.
Direct evidence from observation of independent electrochemical activity of the redox cofactor comprising the active site in the absence of substrate the so called non turn over conditions.
Tight electronic coupling of proteins to electrodes is essential for efficient direct electron transfer det across the bio organic interface. In particular the long standing puzzle of whether enzymatic glucose sensing involves an enzyme direct electron transfer process was studied. Rational modulation of this coupling depends on in depth understanding of the intricate properties of interfacial det. Direct electron transfer det means an exchange of electrons between the cofactor of a redox active enzyme oxidoreductase or a redox protein and an electrode transducer in the absence of redox mediators.