Bioluminescence Resonance Energy Transfer. Depending on the placement of the labels this permits the analysis of conformational changes within. Bioluminescence resonance energy transfer bret is a transfer of energy between a luminescence donor and a fluorescence acceptor.
Bioluminescence resonance energy transfer bret has become a widely used technique to monitor proteinprotein interactions. The energy transfer occurs when the protein tags are in close proximity as described. The distance required for the resonance energy transfer to occur less than 10nm correlates well with most biological interactions making bret ideal.
A bioluminescence resonance energy transfer bret assay system with a bioluminescent enzyme and gfp fusion proteins.
Massoud and sanjiv s. A growing number of tools now allow live recordings of various signaling pathways and protein protein interaction dynamics in time and space by ratiometric measurements such as bioluminescence resonance energy transfer bret imaging. Therefore bret avoids some of the problems inherent in fluorescence resonance. Bret or bioluminescence resonance energy transfer is a cell based assay for studying protein protein interactions.